A class of small non-coding endogenous RNAs known as microRNAs (miRNAs) plays a major role in post-transcriptional gene regulation during plant development and plant responses to biotic and abiotic stresses. It has been suggested that the miRNA pathway contributes to pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), which refers to a basal defense response upon recognition of certain pathogenic elements. To date, the miRNA defense responses in Arabidopsis, rice, and a broad plant host range infected by pathogenic fungus have been evaluated, but none of these studies have been performed on the interaction of grapevine–trunk disease (GTD) pathogens. In this study, we explored di_erentially expressed miRNAs in response to Diaporthe eres and Diaporthe bohemiae infection in Vitis vinifera cv. Chardonnay under in vitro conditions. We used computational methods to predict putative miRNA targets in order to explore the involvement of possible pathogen response pathways. We identified 136 known and 41 new miRNA sequence variants, likely generated through post-transcriptional modifications. In the Diaporthe eres treatment, 61 known and 17 new miRNAs were identified while in the Diaporthe bohemiae treatment, 101 known and 21 new miRNAs were revealed. Our results contribute to further understanding the role miRNAs play during plant pathogenesis, which is possibly crucial in understanding disease symptom development in grapevines infected by D. eres and D. bohemiae.
This study has been carried out by David Gramaje from the BIOVITIS group at the ICVV in collaboration with researchers at Mendel University (Czech Republic) and the University of Agriculture in Krokow (Poland).
This study has been recently published in Genes journal. More information: