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Research Services

INSTRUMENTAL ANALYSIS
Staff:
RESEARCH SERVICES - Staff

Service Manager
Miguel Ángel Fernández Recio
PhD in Chemistry
CSIC R&D&I Technician
941053031 | mirecio@icvv.es

PTA Technician
Cristina Moreta Sánchez
PhD in Chemistry
CSIC Higher graduate
941053031 | cristina.moreta@icvv.es

PTA Technician
Nuria Pérez del Notario García
PhD in Chemistry
CSIC Higher graduate
941053031 | nuria.perezdelnotario@icvv.es

Main duties

- Analytical services to ICVV and external researchers.

- Advice on the different available analytical techniques.

- Centralized laboratory and analytical equipment maintenance.

- Cryogenic and compressed gases facilities management.

- Dangerous wastes management.

- Purified water facilities management.
 

Equipment:

Currently available equipment:

Equipment

UHPLC-DAD-ESI/APCI-MS/MS

Shimadzu Nexera UHPLC (ultra high pressure liquid chromatography) with photodiode array detector (DAD), coupled to an atmospheric pressure ionization source (ESI and APCI) ABSciex 3200QTRAP® triple quadrupole/ion trap mass spectrometer.

Equipment
UHPLC-DAD-ESI/APCI/ASAP-HRMS/MS
Waters Acquity I-Class UHPLC (ultra high pressure liquid chromatography) chromatograph with photodiode array detector (DAD), coupled to a Waters SYNAPT XS HDMS high resolution quadrupole/Time of Flight (qToF) mass spectrometer equipped with an ionic mobility system (IMS) and several ionization sources: atmospheric pressure (ESI and APCI) and solids (ASAP).
UHPLC-DAD-FLD
Shimadzu Nexera UHPLC (ultra high pressure liquid chromatography) with photodiode array detector (DAD) and fluorescence detector(FLD).
HPLC-RID-LSD
Shimadzu Prominence UFLC (ultra fast liquid chromatography) with refraction index detector (RID) and evaporative light scattering detector (ELSD).
GC-MS
Agilent 7890 gas chromatograph, with SSL and PTV injectors, including a thermal desorption system, coupled to an Agilent 7000C triple quadrupole mass spectrometer Gerstel MPS automatized sample injection system (SPME, liquids, Headspace, Twister).
SemiPrep-HPLC:
Gilson semipreparative HPLC chromatograph with photodiode array detector (DAD) and Gilson GX271 automatized fraction injection/collection auto sampler.
SPE
Gilson GX271 automatized solid phase extraction system.
Available skills

- Metabolomic, qualitative and quantitative analysis of complex mixtures by means of chromatographic separation (LC and GC). Detectors: DAD and mass spectrometer (MS, MS2, MS3 , HR, IMS).

- Metabolite identification by High Resolution Mass spectrometry (HRMS).

- Offered provisions:

  • • Polyphenol (anthocyanins, phenolic acids, flavonols, flavanols, stilbenes, lignans) LC analysis.
  • • Biological metabolites (amino acids, amines, polyphenol precursors) LC analysis
  • • Volatile compounds (acids, alcohols, aromatic compounds) GC analysis.
  • • Short chain fatty acids (SCFA) GC analysis

- Handling of different matrixes: samples of plant origin (fermented product, juice, pulp, skin, seed) and samples of animal origin (serum, plasma, urine, faeces).

- Development of chromatographic methods.

- Technical advice and training to the ICVV internal users regarding equipment data treatment software handling: Analyst ®, Multiquant ® and OS-MQ ® (Sciex 3200QTRAP), Progenesis QI ® (Waters SYNAPT HS HDMS), Lab Solution ® (Shimadzu NEXERA), LC Solution ® (Shimadzu PROMINENCE), Mass Hunter Qualitative Analysis ® and Mass Hunter Quantitative Analysis ® (Agilent 7000C GCMS), TRILUTION ® LC (semiprep-HPLC) and TRILUTION ® LH (SPE).

- The provision and fees catalogue for ICVV users is available on the following link: ICVV Internal Users Fees

- The ICVV Instrumental Analysis Service comprehensive provision and fees catalogue for CSIC institutes (including ICVV) as well as other CSIC external centres and companies is available on the following link: ICVV Users Fees

- The CSIC Institutes comprehensive provision and fees catalogue is located in CSIC institutes personnel Intranet on the following link: Catálogo de prestaciones del CSIC

- On-line analysis application form (on build).